Описан улучшенный вариант синтеза гаптена на основе холекальциферола и его активного метаболита 25-гидроксихолекальцеферола. Оптимизирована методика получения высокомолекулярных иммуногенных конъюгатов производных витамина D3 с бычьим сывороточным альбумином, а также конъюгатов с пероксидазой хрена для прямого ИФА. Проведена иммунизация кроликов, выделены поликлональные антитела к 25-гидроксихолекальцеферолу, которые испытаны в иммуноферментном анализе. Для повышения точности метода оптимизирован процесс пробоподготовки, включающий высвобождение витамина D3 и его активных метаболитов из комплексов с витамин D-связывающим белком.
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